Event Details
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Validation of an Er,Cr:YSGG Laser-Assisted Endodontic Protocol using a Rapid, Real-Time ATP Assay Test
Feb 13, 2025
Virtual
Details
The identification of intracanal biologic activity is not only an accurate indicator of the readiness of the root canal system to be obturated, but it also validates the endodontic protocol used. The ATP assay detection method will be presented with data validating an Er,Cr:YSGG laser-assisted protocol for root canal disinfection.
Bacterial testing of root canal systems in patients has traditionally presented with many difficulties. The culture technique never gained wide acceptance as the procedure is arduous and time consuming. DNA-based identification such as polymerase chain reaction suffers from high false positive readings by detecting DNA from dead bacterial cells. Extrapolating data from research in extracted teeth, with artificially created biofilm, to the clinical treatment of patients is fraught with potential miscalculations and erroneous inferences. While this research can provide valuable information as to the efficacy of different endodontic protocols, it does not take in to account the restrictions encountered while treating patients.
A newly introduced ATP assay method provides rapid identification of organic residues in root canal systems by detecting ATP, as well as its degradation products ADP and AMP, using a combination of luminescent reactions. The resulting luminescence is measured within 10 seconds and displayed as a 0 – 100 Endoscore, with low scores indicating less cellular (bacterial) contamination.
The rapid identification of biologic activity within canals not only allows dentists in real time to assess, and if necessary, repeat or modify their endodontic protocol, but it also is an accurate indicator of the readiness of the root canal system to be obturated at that visit.
This presentation will review the ATP assay detection method and present data validating an Er,Cr:YSGG laser-assisted endodontic protocol for the cleaning and disinfection of root canal systems. Clinical cases will be presented illustrating the role that the ATP assay test can play in the management of different clinical situations.
Release date: 2025-02-13 | Expiration date: 2028-02-13